Regulatory
Tatprom

Part:BBa_K562000:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)

Eco_Ptat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The clone carries a BamHI restriction site at its 3' end (increasing the length of the part to 103 bp). If you use this site for cloning, and place a BamHI site (or similar 6-nucleotide site with compatible ends) immediately upsteam of the ATG start codon of your gene of interest, then this places your ATG in exactly the correct position to be initiated from the tatA RBS within the promoter region.


Source

This is from the Escherichia coli K-12 MG1655 genomic sequence.

References

Jack RL, Sargent F, Berks BC, Sawers G, Palmer T. Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. J Bacteriol. 2001 Mar;183(5):1801-4.